Invitrogen

p23 Monoclonal Antibody (JJ3)

Name: p23 Monoclonal Antibody (JJ3)
Brand: Invitrogen
SKU: MA3-414

高级验证

14个已发表图片

34 篇参考文献

查看其他18个p23抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

p23 Antibody in Immunocytochemistry (ICC/IF)

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p23 Antibody (MA3-414) in ICC/IF

Immunofluorescent analysis of p23 using Anti-p23 Monoclonal Antibody (JJ5) (Product # MA3-414) shows staining in Hela Cells. p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 (Product # MA3-414) at a dilution of 1:500 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat ... View More

p23 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

p23 Antibody in Immunoprecipitation (IP)

p23 Antibody in RNA Immunoprecipitation (RIP)

p23 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

p23 Antibody in Immunohistochemistry (IHC)

p23 Antibody in Proximity Ligation Assay (PLA) (PLA)

Product Image Gallery

p23 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. T-47D cells were transfected with p23 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-p23 Monoclonal Antibody (JJ3) (Product # MA3-414). {KD}

产品信息

MA3-414

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看20篇已发表文章 20篇已发表文章

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:200-1:2,000

查看4篇已发表文章 4篇已发表文章

流式细胞分析 (Flow)

1/100

免疫沉淀 (IP)

Assay-dependent

查看5篇已发表文章 5篇已发表文章

Proximity Ligation Assay (PLA) (PLA)

查看1篇已发表文章 1篇已发表文章

RNA免疫共沉淀 (RIP)

Assay-dependent

产品规格

种属反应

Chicken, Guinea pig, Human, Mouse, Rabbit, Rat

已发表种属

Human, Mouse, Rabbit, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

JJ3

抗原

Recombinant human p23 protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2175197

产品详细信息

MA3-414 detects p23 from chicken, guinea pig, human, rat, mouse and rabbit samples.

MA3-414 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 19 or 23 kDa protein representing p23 from rabbit reticulocyte lysate. MA3-414 can be used to immunoprecipitate both free and complexed p23.

The MA3-414 antigen is recombinant human p23.

靶标信息

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Co chaperone p23; cPGES; cytosolic prostaglandin E synthase; Cytosolic prostaglandin E2 synthase; Hsp90 co chaperone; Hsp90 co-chaperone; p23 cochaperone; Progesterone receptor complex p23; Prostaglandin E synthase 3; prostaglandin E synthase 3 (cytosolic); prostaglandin-E synthase 3; ptges3 {ECO:0000250|UniProtKB:Q15185}; Sid 3177; telomerase binding protein, p23; Telomerase-binding protein p23; unactive progesterone receptor, 23 kD

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基因别名: 5730442A20Rik; CPGES; P23; Ptges; PTGES3; RGD1561913; Sid3177; TEBP