Rockland

p28ING5 Polyclonal Antibody

Name: p28ING5 Polyclonal Antibody
Brand: Rockland
SKU: 600-101-285

查看其他10个ING5抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

图: 1 / 2

p28ING5 Antibody (600-101-285) in WB

Western blot analysis is shown using Rocklands Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. Comparison to a molecular weight marker (not shown) indicates a single band of ~45.0 kDa corresponding to the expected molecular weight for the recombinant protein. The blo... View More

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Immunogen image

Immunogen Image p28ING5 Polyclonal Antibody

产品信息

600-101-285

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:10,000

酶联免疫吸附实验 (ELISA)

1:4,000-1:16,000

免疫沉淀 (IP)

1:500-1:1,000

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 125-150 of Human p28 ING5 protein (Inhibitor of growth family, member 5).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.52 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reactivity occurs against human p28 ING5 protein. The sequence is present in both isoforms 1 and 2. However, 100% homology is on record for this protein from human and chimpanzee. Cross reactivity with p28 ING5 protein from frog, mouse, rat and chicken may not occur as sequence homology varies by one or more amino acids residues in this sequence as indicated by BLAST analysis. Reactivity with p28 ING5 proteins from other sources is not known.

靶标信息

ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway.

仅用于科研。不用于诊断过程。未经明确授权不得转售。