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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • p300 Antibodies

          Invitrogen

          p300 Monoclonal Antibody (RW128)

          高级验证
          4 篇参考文献
          查看其他16个p300抗体

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          Cite p300 Monoclonal Antibody (RW128)

          • 抗体检测数据 (8)
          • 高级验证 (2)
          p300 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          p300 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 10

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          p300 Antibody (MA1-16608) in ICC/IF

          Immunofluorescence analysis of p300 Monoclonal Antibody (RW128) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with p300 Monoclonal Antibody (RW128) (Product # MA1-16608) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Ale... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          查看产品

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          p300 Antibody in Immunocytochemistry (ICC/IF)
          p300 Antibody in Immunocytochemistry (ICC/IF)
          p300 Antibody in Immunocytochemistry (ICC/IF)
          p300 Antibody in Immunocytochemistry (ICC/IF)
          p300 Antibody in Western Blot (WB)
          p300 Antibody in Western Blot (WB)
          p300 Antibody in Flow Cytometry (Flow)
          p300 Antibody in Flow Cytometry (Flow)
          p300 Antibody
          p300 Antibody
          p300 Monoclonal Antibody (RW128)

          产品信息

          MA1-16608

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:250-1:500
          查看3篇已发表文章 3篇已发表文章

          免疫细胞化学 (ICC/IF)

          2-5 µg/mL
          -

          流式细胞分析 (Flow)

          1 µg/mL
          -

          免疫沉淀 (IP)

          1:10-1:500
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Human, Mustelid, Mouse, Non-human primate, Rat

          已发表种属

          Human, Mouse

          宿主/亚型

          Mouse / IgG1, kappa

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          RW128

          抗原

          Fusion protein containing residues 1572-2371 of human KAT3B/p300.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Protein A

          保存液

          PBS

          内含物

          0.02% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_568667

          产品详细信息

          Suggested positive control: HeLa nuclear extract.

          靶标信息

          EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: E1A-associated protein p300; E1A-binding protein, 300kD; Histone acetyltransferase p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; p300 HAT; Protein 2-hydroxyisobutyryltransferase p300; Protein lactyltransferas p300; Protein propionyltransferase p300

          查看更多 收起

          基因别名: A430090G16; A730011L11; EP300; KAT3B; P300; p300 HAT; RSTS2

          查看更多 收起

          UniProt ID: (Human) Q09472, (Mouse) B2RWS6

          查看更多 收起

          Entrez Gene ID: (Human) 2033, (Mouse) 328572, (Rat) 170915

          查看更多 收起

          功能
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding p53 binding DNA binding chromatin binding damaged DNA binding transcription coactivator activity histone acetyltransferase activity lysine N-acetyltransferase activity, acting on acetyl phosphate as donor protein binding beta-catenin binding protein C-terminus binding transcription factor binding zinc ion binding acetyltransferase activity transferase activity, transferring acyl groups chromatin DNA binding protein complex binding activating transcription factor binding peptide N-acetyltransferase activity nuclear hormone receptor binding glucocorticoid receptor binding peroxisome proliferator activated receptor binding bHLH transcription factor binding SMAD binding androgen receptor binding mitogen-activated protein kinase binding NF-kappaB binding pre-mRNA intronic binding protein antigen binding RNA polymerase II transcription factor binding core promoter proximal region DNA binding transcription factor activity, sequence-specific DNA binding transcription cofactor activity antigen binding transferase activity protein kinase binding metal ion binding histone modifying enzyme
          参与通路
          negative regulation of transcription from RNA polymerase II promoter response to hypoxia somitogenesis liver development positive regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway transcription-coupled nucleotide-excision repair regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein acetylation internal protein amino acid acetylation apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response Notch signaling pathway nervous system development heart development skeletal muscle tissue development memory circadian rhythm organ morphogenesis regulation of autophagy positive regulation of glycoprotein biosynthetic process macrophage derived foam cell differentiation positive regulation of cell death positive regulation of muscle atrophy viral process N-terminal peptidyl-lysine acetylation internal peptidyl-lysine acetylation B cell differentiation platelet formation lung development response to cobalt ion positive regulation of protein binding positive regulation of type I interferon production positive regulation of collagen biosynthetic process positive regulation of protein import into nucleus, translocation response to tumor necrosis factor cellular response to UV positive regulation of histone acetylation cellular response to drug megakaryocyte development cellular response to trichostatin A intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of DNA binding protein kinase B signaling response to estrogen positive regulation by host of viral transcription histone H3 acetylation histone H4 acetylation histone H2B acetylation fat cell differentiation response to ethanol positive regulation of translation positive regulation of axon extension positive regulation of cell size positive regulation of gene expression, epigenetic positive regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter digestive tract development positive regulation of protein secretion protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity response to calcium ion regulation of cell cycle regulation of angiotensin metabolic process positive regulation of sarcomere organization regulation of androgen receptor signaling pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia protein-DNA complex assembly cellular response to hydrogen peroxide response to fatty acid cellular response to retinoic acid cellular response to cAMP cellular response to glucose stimulus cellular response to mineralocorticoid stimulus cellular response to dexamethasone stimulus regulation of tubulin deacetylation regulation of cellular response to heat regulation of signal transduction by p53 class mediator beta-catenin-TCF complex assembly cellular response to nerve growth factor stimulus negative regulation of miRNA metabolic process transcription, DNA-templated cell cycle response to glucose positive regulation of gene expression histone acetylation cell differentiation positive regulation of cell growth negative regulation of cellular metabolic process positive regulation of cellular metabolic process response to drug positive regulation of transcription, DNA-templated rhythmic process cartilage development negative regulation of cell death response to dexamethasone positive regulation of protein acetylation regulation of nucleic acid-templated transcription response to organic cyclic compound response to retinoic acid response to hydrogen peroxide cellular response to antibiotic cellular response to organic cyclic compound
          It has to be done as per old AB suggested Products section.
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