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Agrisera
pHRed Polyclonal Antibody
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产品信息
AS16-3828
产品规格
种属反应
Tag
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant part of pHRed of Chlamydomonas reinhardtii.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: pHRed
靶标信息
pHRed is a genetically encoded, cytosolically expressed fluorescent protein sensor engineered from the monomeric red fluorescent protein mKeima and is typically introduced into mammalian cells as a synthetic coding sequence for live-cell imaging; when expressed without targeting motifs it reports bulk intracellular (cytosolic) pH, and when fused to organelle-localization sequences it can be used to measure compartment-specific pH (e.g., mitochondria). Structurally, pHRed is a single polypeptide fluorescent protein that folds into the characteristic beta-barrel (GFP-like) architecture surrounding an autocatalytically formed chromophore, and it behaves as a ratiometric indicator with two excitation maxima (approximately 440 and 585 nm) and red emission (approximately 610 nm). Functionally, pHRed transduces changes in protonation state into optical readouts: the excitation ratio changes with pH with an apparent pKa near 6.6 and a large dynamic range, enabling quantitative monitoring of physiologic pH shifts in living cells; in addition to intensity-based ratiometry, pHRed exhibits a pH-dependent fluorescence lifetime that can be exploited for lifetime imaging approaches to track energy-dependent pH changes in the cytosol or mitochondria.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: pHRed; pHRed biosensor
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