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Invitrogen
14-3-3 beta/zeta Monoclonal Antibody (4E2)
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14-3-3 beta/zeta Antibody (MA1-25665) in ICC/IF
Immunofluorescence analysis of 14-3-3 protein beta/zeta was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with 14-3-3 beta/zeta Monoclonal Antibody (4E2) (Product # MA1-25665) at 1:50 dilution in 0.1% BSA,... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA1-25665
产品规格
宿主/亚型
Mouse
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
4E2
抗原
Lysed, transformed, human amnion cells.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
50 µg
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
Recommended positive controls: HeLa cell lysate.
靶标信息
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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