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Invitrogen
53BP1 Polyclonal Antibody, DyLight™ 488
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FIGURE: 1 / 3
53BP1 Antibody (PA5-22759) in WB
Western blot was performed using Anti-53BP1 Polyclonal Antibody, DyLight 488 (Product # PA5-22759) and a ~270kDa band corresponding to TP53-binding protein 1 was observed across cell lines tested. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), MDA-MB-231 (Lane 3), MCF7 (Lane 4), U-2 OS (Lane 5), K-562 (Lane 6), U-87 MG (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® ... View More
Product Details
PA5-22759
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bat,
Bovine,
Dog,
Human,
Mouse,
Rodent,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residues 1925 and the C-terminus (residue 1972) of human 53BP1.
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
50mM sodium borate
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Human samples have been tested in Western Blot and ICC/IF, and mouse samples have been tested in ICC/IF only.
Suggested positive control: U205, 293T, and MO59K cell lysates and MEF lysates.
Predicted cross-reactivity based on sequence identity: Chimpazee/Equine/Feline/Gibbon/Gorilla/Hamster/Marmoset/Orangutan/Panda/Porcine/Rabbit/Sheep (100%).
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 53BP1; FLJ41424; MGC138366; murine p53-binding protein; p53-binding protein 1; TP53-binding protein 1; transformation related protein 53 binding protein 1; transformation related protein 53 binding-protein 1; tumor protein 53-binding protein, 1; tumor protein p53-binding protein, 1; tumor suppressor p53-binding protein 1
Gene Aliases: 53BP1; m53BP1; p202; p53BP1; TDRD30; TP53; TP53BP1; Trp53bp1
UniProt ID: (Human) Q12888, (Mouse) P70399
Entrez Gene ID: (Dog) 478274, (Human) 7158, (Bovine) 509111, (Rat) 296099, (Mouse) 27223
RNA polymerase II activating transcription factor binding
RNA polymerase II transcription cofactor activity
p53 binding
DNA binding
protein binding
methylated histone binding
molecular_function
damaged DNA binding
telomeric DNA binding
sequence-specific DNA binding
transcription factor binding
DNA metabolism protein
DNA damage checkpoint
double-strand break repair via nonhomologous end joining
transcription, DNA-templated
cellular response to DNA damage stimulus
protein sumoylation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
cellular response to X-ray
DNA repair
transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
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