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Invitrogen
7-Ketocholesterol Monoclonal Antibody (7E1), APC
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7-Ketocholesterol Antibody (MA5-45793) in WB
Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol protein. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (2.0 µg). Lane 4: 7-ketocholesterol-BSA (0.5 µg). Lane 5: 7-ketocholesterol-BSA (2.0 µg). Blocking: 5% Skim Milk in TBST. Samples were incubated with 7-Ketocholesterol monoclonal antibody (Product # MA5-45793) at 1:1,000 for 2 hours at RT, followed by Goat Anti-Mouse IgG: HRP at 1:2,000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predic... View More
产品信息
MA5-45793
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
7E1
抗原
Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
A 1:1,000 dilution of MA5-45793 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for 7-ketocholesterol modified proteins.
靶标信息
7-Ketocholesterol is a major oxidation product of cholesterol found in human atherosclerotic plaque and is more atherogenic than cholesterol in some animal studies. 7-Ketocholesterol can inhibit cholesterol 7alpha-hydroxylase, the rate-limiting step in bile acid biosynthesis, as well as strongly inhibiting HMG-CoA reductase, the rate-limiting enzyme in cholesterol biosynthesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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