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Invitrogen
ACTA1 Polyclonal Antibody
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FIGURE: 1 / 9
ACTA1 Antibody (PA5-78715) in IHC (P)
Immunohistochemistry (Paraffin) analysis of Actin in paraffin-embedded section of human testicular germ cell tumor tissue using Actin Polyclonal Antibody (Product # PA5-78715). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilut... View More
Product Details
PA5-78715
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (277-308aa HETTYNSIMKCDIDIRKDLYANNVMSGGTTMY).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat heart tissue, rat brain tissue, rat kidney tissue, mouse heart tissue, mouse brain tissue, mouse spleen tissue, mouse kidney tissue, human Hela whole cell, human HepG2 whole cell, human 293T whole cell, human A431 whole cell. IHC: human liver cancer tissue, human testicular germ cell tumor tissue, mouse heart tissue, rat heart tissue.
Target Information
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: actin alpha 1; Actin, alpha skeletal muscle; Alpha-actin-1; nemaline myopathy type 3
Gene Aliases: AA959943; ACTA; Acta-2; ACTA1; Acts; Actsk-1; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; SHPM
UniProt ID: (Human) P68133, (Mouse) P68134, (Rat) P68136
Entrez Gene ID: (Human) 58, (Mouse) 11459, (Rat) 29437
muscle contraction
response to mechanical stimulus
response to extracellular stimulus
response to lithium ion
positive regulation of gene expression
cell growth
muscle filament sliding
skeletal muscle thin filament assembly
skeletal muscle fiber adaptation
response to steroid hormone
skeletal muscle fiber development
mesenchyme migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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