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Invitrogen
AKAP12 Polyclonal Antibody
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AKAP12 Antibody (PA1-29372) in WB
Western Blot using AKAP12 Polyclonal Antibody (Product # PA1-29372). Total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyzed. The membrane was probed with 5 µg/mL of pre-immune serum or 5 µg of AKAP12 Polyclonal Antibody (Product # PA1-29372) (3 second exposure shown). The blot was then washed and probed with monoclonal anti-sheep IgG antibody coupled to horseradish peroxidase 1:10,000, and detected with luminol.
产品信息
PA1-29372
应用
建议稀释比
已发表文章
产品规格
种属反应
Rat
已发表种属
C. elegans
宿主/亚型
Sheep
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Rat AKAP12 fragment corresponding to amino acid 620-1596.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
PBS
内含物
0.08% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-29372 detects SSecks from rat samples.
靶标信息
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: A kinase (PRKA) anchor protein (gravin) 12; A-kinase anchor protein 12; AKAP-12; SSeCKS, Gravin, AKAP250
基因别名: Akap12; AKAP12A; AKAP12B; AKAP12G
UniProt ID: (Rat) Q5QD51
Entrez Gene ID: (Rat) 83425
