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Invitrogen
AKAP2 Polyclonal Antibody
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AKAP2 Antibody (PA5-78749) in WB
Western blot analysis of AKAP2 using AKAP2 Polyclonal Antibody (Product # PA5-78749). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates. Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-78749
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human AKAP2 (813-852aa ETHKSKRRERMDDSSVLEATRVNRRKSALALRWEAGIYAN).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human PC-3 whole cell, mouse lung tissue.
靶标信息
PALM2-AKAP2 is a fusion/readthrough protein associated with the PALM2AKAP2 locus that contains regions related to both PALM2 (paralemmin-2) and AKAP2 (A-kinase anchoring protein 2). The AKAP2-derived portion is linked to A-kinase anchoring functions that help organize protein kinase A (PKA) signaling within cells, while the PALM2-derived portion is related to membrane-associated paralemmin proteins. PALM2-AKAP2 should therefore be distinguished from PALM2 or AKAP2 alone, although antibodies targeting shared regions may cross-react with the corresponding individual protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: A kinase (PRKA) anchor protein 2; A-kinase anchor protein 2; A-kinase anchoring protein 2; AKAP expressed in kidney and lung; PALM2-AKAP2 fusion; PALM2-AKAP2 readthrough; Palm2-Akap2 readthrough transcript; paralemmin 2; paralemmin A kinase anchor protein; protein kinase A anchoring protein 2; Protein kinase A-anchoring protein 2; protein kinase A2; unnamed protein product
基因别名: 53-H12; AA959716; AI649048; AKAP-2; AKAP-KL; AKAP2; AKAPKL; B130066I24; B230340M18Rik; Gm20459; MISP2; PALM2; PALM2-AKAP2; Palm2Akap2; pEN70; PRKA2; R75368
UniProt ID: (Human) Q9Y2D5, (Mouse) O54931
Entrez Gene ID: (Human) 445815, (Mouse) 677884
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