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AKINB2/SNF1-related protein kinase regulatory subunit beta-2 Polyclonal Antibody
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AKINB2/SNF1-related protein kinase regulatory subunit beta-2 Antibody (AS09-462) in WB
25 µg of empty vector (1), p CDNA 3.1 – HA- tagged AKIN-beta 1 (2), p CDNA 3.1 – HA- tagged AKIN-beta 2 (3), 50 µg of total protein from Arabidopsis thaliana leaves (4) had been homogenized into 50 m M Na PO 4 p H 7.5,20 m M KCl, 0.5 M sucrose, 0.2 m M PMSF, 10 m M DTT and protease inhibitor cocktail (Sigma, P 9599). Extracts were separated on 10%Nu Page Bis-Tris Novex SDS PAGE (Invitrogen) gels followed by... View More
产品信息
AS09-462
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-2.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
200 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
AKINB2 encodes the Arabidopsis thaliana SNF1-related protein kinase (SnRK1) regulatory subunit beta-2, a non-catalytic component that associates with SnRK1 alpha catalytic and gamma regulatory subunits to form heterotrimeric kinase complexes that act as central metabolic sensors. The AKINB2 protein is characterized by the conserved AMPK/SNF1 beta-subunit interaction module (including regions that mediate binding to partner subunits and assembly of the holoenzyme), supporting its adaptor-like role in complex formation and target engagement rather than intrinsic enzymatic activity. In plants, SnRK1 complexes are activated under energy limitation and diverse environmental stresses to reprogram transcription and metabolism, promoting catabolic pathways and restricting energy-consuming biosynthesis; consistent with this framework, AKINB2 is thought to contribute to SnRK1 complex integrity, subcellular distribution and/or substrate specificity, thereby tuning downstream responses that coordinate carbohydrate and lipid metabolism and broader stress adaptation in vivo.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AKINbeta2; KINB2
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