Invitrogen

AKT2 Polyclonal Antibody

Name: AKT2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-13762

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1 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

说明书

实验方案

技术支持

AKT2 Antibody in Immunocytochemistry (ICC/IF)

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AKT2 Antibody (PA5-13762) in ICC/IF

Immunofluorescent analysis of C2C12 cells stained with an AKT2 polyclonal antibody (Product # PA5-13762). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with an AKT2 polyclonal antibody (Product # PA5-13762) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (... View More

AKT2 Antibody in Immunohistochemistry (IHC)

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AKT2 Antibody — FIG. 5. Rictor/mTORC2 is activated in type 1 diabetes. OVE26 mice (17 weeks old) were treated with phosphorothioated sense (S) or AS oligonucleotides for Rictor (90 ng*g body wt - 1 *day - 1 ), administered subcutaneously by an ALZET osmotic minipump for 1 month. Mice in the control groups received saline vehicle also administered subcutaneously by an ALZET osmotic minipump. Glomeruli were isolated from the kidneys of the four groups of mice ( n = 5): group 1, FVB control mice; group 2, OVE26 mice; and groups 3 and 4, OVE26 mice treated with either S or AS. (A) Relative mRNA levels of Rictor. (B) Relative mRNA levels of Akt1. (C) Relative mRNA levels of Akt2. (D) Representative Western blot of Rictor, phospho-Ser 473 Akt, phospho-Thr 308 Akt, phospho-Thr 346 NDRG1, Akt1, Akt2, and beta-actin. beta-actin was included as a control for loading and the specificity of change in protein expression. (E) Histograms showing quantitation of Rictor/beta-actin from five different mice in each group. (F) Histograms showing quantitation of p-Akt (Ser473)/beta-actin results from five different mice in each group. (G) Histograms showing quantitation of p-Akt (Thr308)/beta-actin results from five different mice in each group. (H) Histograms showing quantitation of p-NDRG1 (Thr346)/beta-actin results from five different mice in each group. (I) Histograms showing quantitation of Akt1/beta-actin results from five different mice in each group. (J) Histograms showing quantitation of Akt2/beta-acti {TM}

产品信息

PA5-13762

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:10-1:50

免疫细胞化学 (ICC/IF)

1:10-1:50

产品规格

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 452-481 amino acids from human AKT2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Lot-specific

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2225158

产品详细信息

This antibody is predicted to react with mouse and rat based on sequence homology.

靶标信息

AKT2 is a phosphoinositide-dependent serine/threonine protein kinase that is enriched in insulin responsive tissues and has been implicated in the metabolic actions of the hormone. Akt is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

AKT2 Polyclonal Antibody

AKT2 Antibody (PA5-13762) in ICC/IF

产品信息

PA5-13762

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

保存条件

运输条件

RRID

产品详细信息

靶标信息

性能保障

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