Invitrogen

ALK Monoclonal Antibody (SP8)

Name: ALK Monoclonal Antibody (SP8)
Brand: Invitrogen
SKU: MA5-14528

4 篇参考文献

查看其他48个ALK抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

ALK Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

ALK Antibody (MA5-14528) in ICC/IF

Immunofluorescent analysis of ALK (Anaplastic Lymphoma Kinase)/p80 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an ALK/p80 monoclonal antibody (Product # MA5-14528) in 3% BSA-PBS at a dilution of 1:10 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST... View More

ALK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image ALK Monoclonal Antibody (SP8)

产品信息

MA5-14528

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

1:10-1:100

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

SP8

抗原

Recombinant fragment within ALK aa 1350-1500. The exact sequence is proprietary.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

tissue culture supernatant/TBS, pH 7.5, with 1% BSA

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_10982161

产品详细信息

MA5-14528 targets ALK (Anaplastic Lymphoma Kinase) / p80 in IHC (P) and ICC/IF applications and shows reactivity with Human and Mouse samples.

The MA5-14528 immunogen is recombinant protein corresponding to a region, which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript.

靶标信息

Anaplastic Lymphoma Receptor Tyrosine Kinase (ALK) belongs to the insulin receptor superfamily. It is vital for brain development. Mutations, rearrangements, and amplifications in the ALK gene have been found in tumors, including anaplastic large cell lymphoma, neurblastoma, and non-small cell lung cancer. Chromosomal rearrangement is the most common genetic alteration. The translocation creates a fusion gene consisting of the ALK gene and the nucleophosmin gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: ALK tyrosine kinase receptor; ALK/NPM1 fusion gene; Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki1; Anaplastic Lymphoma Kinase p80; CD246; CD246 antigen; EC 2.7.10.1; kinase ALK; mutant anaplastic lymphoma kinase; npm-alk; p80; TFG/ALK

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基因别名: ALK; CD246; NBLST3; Tcrz

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UniProt ID: (Human) Q9UM73, (Mouse) P97793

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Entrez Gene ID: (Human) 238, (Mouse) 11682

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功能