Invitrogen

AMHR2 Polyclonal Antibody

Name: AMHR2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-13901

高级验证

1个已发表图片

1 篇参考文献

查看其他12个AMHR2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 3

AMHR2 Antibody (PA5-13901) in WB

Western blot analysis of AMHR2 (arrow) using an AMHR2 polyclonal antibody (Product # PA5-13901) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the AMHR2 gene (Lane 2).

Product Image Gallery

AMHR2 Antibody — AMH and AMHR2 regulate survival signaling in NSCLC (A) Schematic of TGF-β, BMP and AMH signaling. (B) qRT-PCR for AMH, normalized to total levels of AMH in testis (positive control), for two NSCLC cell lines (A549, H1299), two ovarian cancer cell lines (O5 = OVCAR5, O8 = OVCAR8) and normal lung tissue. (C, D) Western blots indicating depletion of AMH (C) or AMHR2 (D) for two independent siRNAs in A549 cells, with quantification of results normalized to GAPDH loading controls. (E) FACS using AMHR2-specific antibodies and non-permeabilized (blue; membranous (M)) or permeabilized (green; total (T)) cells after depletion of AMHR2. Averaged median fluorescence intensity (MFI) values from multiple independent experiments for each condition normalized to membraneous AMHR2 (control (C) siRNA) are show. The percentage of membranous AMHR2 (gray) is the MFI for M divided by the MFI for T. (F) Representative Western blots and quantifications for total and phosphorylated SMAD1/5/8 following siRNA depletion of AMH and AMHR2 in A549 cells. (G) Representative Western blots for total and phosphorylated SMAD1/5/8 following doxycycline (Dox) induced overexpression of AMH in A549 cells. (H) Representative Western blots for total and phosphorylated SMAD1/5/8 following 1 hour stimulation of A549 and SMAT1 cells with recombinant AMH or BMP2 (positive signaling control). (I) Representative Western blots for indicated total and phosphorylated survival-signaling proteins, following siRNA depletion of {KD}

产品信息

PA5-13901

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

产品规格

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 65-91 amino acids from the N-terminal region of human AMHR2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.35 mg/mL

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2226494

产品详细信息

This antibody is predicted to react with mouse based on sequence homology.

靶标信息

The AMH receptor (AMHR or AMHR2) is a serine/threonine kinase with a single transmembrane domain belonging to the family of type II receptors for TGF-beta-related proteins. Anti-Mullerian hormone (AMH) and its receptor are involved in the regression of Mullerian ducts in male fetuses. Male sex differentiation is mediated by 2 discrete hormones produced by the fetal testis. Testosterone, produced by Leydig cells, virilizes the external genitalia and promotes prostatic growth; anti-Mullerian hormone (AMH) results in regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

AMHR2 Polyclonal Antibody

AMHR2 Antibody (PA5-13901) in WB

产品信息

PA5-13901

免疫印迹 (WB)

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

保存条件

运输条件

RRID

产品详细信息

靶标信息

性能保障

随时为您服务

Your items have has been added!