Osenses

AQP0 Polyclonal Antibody

Name: AQP0 Polyclonal Antibody
Brand: Osenses
SKU: OSA00101W-100UL

1 篇参考文献

查看其他7个Aquaporin 0抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

AQP0 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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AQP0 Antibody (OSA00101W-100UL) in IHC (P)

IHC on paraffin sections of human skin tissue using Rabbit antibody to AQP0 (140-180): OSA00101W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained wi... View More

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Immunogen image

Immunogen Image AQP0 Polyclonal Antibody

产品信息

OSA00101W-100UL

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:300-1:2,000

免疫组化（石蜡） (IHC (P))

1:1,000

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

宿主/亚型

Rabbit / Ig

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide from aa 140 -180 of human AQP0 conjugated to blue carrier protein was used as the antigen

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

Conc. Not Determined

保存液

whole serum

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide shares 95% identity with rat, mouse and guinea pig sequences.

Specificity of this antibody: AQP0.

靶标信息

AQP0 is the most abundant endogenous protein in the plasma membrane of lens fiber cells where it functions not only as a water pore, but it is also involved in fiber-fiber adhesion and is crucial for fiber cell structure and organization. AQP0 contains an additional pore constriction, not seen in any other aquaporin structures, which may be responsible for pore gating. The closed AQP0 pore holds just three water molecules, which are spaced too far apart to form hydrogen bonds with each other. The C-terminal domain of AQP0 undergoes extensive post-translational modification, including many truncations, during lens aging due to the actions of m-calpain, proteases or non-enzymatic mechanisms. These truncation sites may be involved in the development of cataracts.

仅用于科研。不用于诊断过程。未经明确授权不得转售。