Invitrogen

ATP1A1 Monoclonal Antibody (464.6)

Name: ATP1A1 Monoclonal Antibody (464.6)
Brand: Invitrogen
SKU: MA1-16731

5个已发表图片

9 篇参考文献

查看其他26个ATP1A1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (10)
已发表图片 (5)

ATP1A1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 15

ATP1A1 Antibody (MA1-16731) in ICC/IF

Immunocytochemistry analysis of ATP1A1 in HEK293 cells. Samples were incubated with ATP1A1 monoclonal antibody (Product # MA1-16731) using a dilution of 10 µg/mL overnight at 4°C followed by anti-mouse DyLight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATP1A1 Antibody in Flow Cytometry (Flow)

ATP1A1 Antibody in Immunohistochemistry (IHC)

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Immunogen image

Immunogen Image ATP1A1 Monoclonal Antibody (464.6)

产品信息

MA1-16731

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200

免疫细胞化学 (ICC/IF)

1:50-1:1,000

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

1:50-1:200

亲和纯化 (AP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, Dog, Fruit fly, Guinea pig, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus, Yeast

已发表种属

Amphibian, C. elegans, Guinea pig, Human, Rat, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

464.6

抗原

Purified Sodium Potassium ATPase Alpha 1 from rabbit renal outer medulla.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2060993

产品详细信息

Do not boil samples prior to Western Blot.

靶标信息

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: (Na+, K+)-ATPase alpha-subunit; ATP1B; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na+ ,K+ -ATPase alpha subunit; Na+ /K+ ATPase alpha 1 subunit; Na+, K+-ATPase beta-subunit precursor; Na+/K+ -ATPase alpha 1 subunit; Na+/K+ ATPase; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na+/K+ transporting alpha 1 polypeptide; Na+K+ ATPase; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; Na/K ATPase alpha 1 subunit; RP4-655J12.1; Sodium Potassium ATPase; sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; sodium/potassium-ATPase alpha-subunit 1; sodium/potassium-transporting ATPase alpha-1 chain; Sodium/potassium-transporting ATPase subunit alpha-1; unnamed protein product

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基因别名: ATP1A1; atp1a1-a; atp1a1-b; atp1a1a.4; Atpa-1; BC010319; K-ATPase; Na+K+ATPase; Nkaa1b