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OriGene
ATP6V1F Monoclonal Antibody (OTI6C11), TrueMAB™
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图: 1 / 3
ATP6V1F Antibody (CF502322) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1F (RC210728, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6. Positive lysates LY418132 (100 µg) and LC418132 (20 µg) can be purchased separately from OriGene.
产品信息
CF502322
产品规格
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
OTI6C11
抗原
Full length human recombinant protein of human ATP6V1F produced in E.coli.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
1 mg/mL
规格
100 µg
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
靶标信息
ATP6V1F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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