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Invitrogen
Actin Monoclonal Antibody (ACTN05 (C4)), Biotin
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Actin Antibody (MA5-11866) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), A549 (Lane 2), COS-7 (Lane 3), MDCK (Lane 4), NIH/3T3 (Lane 5), HT-29 (Lane 6), PC-12 (Lane 7), Neuro-2a (Lane 8), tissue extracts of Mouse Brain (Lane 9) and Rat Skeletal Muscle (Lane 10). The blot was probed with Actin Monoclonal Antibody (ACTN05 (C4)), Biotin (Product # MA5-11866, 1:5000 dilution) and detected by chemiluminescence using Poly-HRP Streptavidin (Product # N200, 1:10000 dilution). A 42 kDa band corresponding to Actin was observed ac... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-11866
应用
建议稀释比
已发表文章
产品规格
已发表种属
Chordate,
Dog,
Fish,
Fruit fly,
Hamster,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat,
Xenopus,
Yeast,
Zebrafish
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
ACTN05 (C4)
抗原
Chicken gizzard actin
偶联物
Biotin
Biotin
Biotin
形式
Liquid
浓度
0.2 mg/mL
规格
100 µg
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody reacts with all six known vertebrate isoforms of actin (MW ~42 kDa), as well as cytoplasmic actin beta and actin gamma. This antibody is highly recommended for monitoring total protein load on Western blots.
Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Suggested positive controls are HeLa cells and skeletal muscle.
靶标信息
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
