Alomone Labs, LTD

Adenosine A3 Receptor Polyclonal Antibody

Name: Adenosine A3 Receptor Polyclonal Antibody
Brand: Alomone Labs, LTD
SKU: AAR-004-200UL

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

Adenosine A3 Receptor Antibody in Immunocytochemistry (ICC/IF)

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Adenosine A3 Receptor Antibody (AAR-004-200UL) in ICC/IF

Expression of AdenosineA3Receptor in a human melanoma cell line - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human melanoma cells (A2058). A, D. Cells were stained using Anti-AdenosineA3Receptor Antibody (#AAR-004), (1:100), followed by goat Anti-rabbit AlexaFluor-555 secondary Antibody . B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 3... View More

Adenosine A3 Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Adenosine A3 Receptor Antibody in Western Blot (WB)

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Immunogen image

Immunogen Image Adenosine A3 Receptor Polyclonal Antibody

产品信息

AAR-004-200UL

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

免疫组化（石蜡） (IHC (P))

1:50

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

(C)KETGAFYGREFKTAK, corresponding to amino acid residues 216-230 of human A3AR, 3rd intracellular loop

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.7 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).

靶标信息

Adenosine is involved in a variety of processes, including the synthesis of urea, the anti-inflammatory response and the inhibition of protein synthesis The adenosine receptors, including adenosine A1-R, adenosine A2A-R, adenosine A2B-R and adenosine A3-R, are integral membrane proteins that are members of the G protein-coupled receptor family. The A1-R protein mediates ureagenesis in a partially calcium-dependent manner. Adenosine is known to mediate coronary vasodilation via the A2A-R receptor. Collagen synthesis and total protein synthesis are inhibited in certain cells by adenosine, acting via the A2B receptors. Activation of the A3-R receptor inhibits the induction of the cytokine TNF alpha and blocks the endotoxin CD14 receptor signal transduction pathway.

仅用于科研。不用于诊断过程。未经明确授权不得转售。