Invitrogen

ApoA1 Polyclonal Antibody

2个已发表图片

2 篇参考文献

查看其他43个ApoA1抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (1)
已发表图片 (2)

ApoA1 Antibody in Western Blot (WB)

ApoA1 Antibody in Western Blot (WB)

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ApoA1 Antibody (PA5-21166) in WB

Western Blot analysis of ApoA1 in chicken liver tissue lysate with ApoA1 Polyclonal Antibody (Product # PA5-21166) at 1 µg/mL .

ApoA1 Antibody in Western Blot (WB)

ApoA1 Antibody in Western Blot (WB)

ApoA1 Antibody in Western Blot (WB)

Product Image Gallery

ApoA1 Antibody in Western Blot (WB) — Western Blot analysis of ApoA1 in chicken liver tissue lysate with ApoA1 Polyclonal Antibody (Product # PA5-21166) at 1 µg/mL .

ApoA1 Antibody in Western Blot (WB) — Figure 6 The purity of EV preparation. (A) Western blot comparing protein levels in three biological replicates of ABEVs (EV) and crude brainstem lysates (BS). Neural cell adhesion molecule (NCAM), a positive marker for EVs released by neural tissue, was enriched in ABEVs. In contrast, negative markers for EVs, namely calnexin (CANX), APOA1, and VDAC, showed either no difference in protein amount between brainstem lysates and ABEVs (CANX and APOA1) or showed a decreased expression in ABEVs (VDAC). Both procaspase-3 (PC3) and cleaved caspase-3 (CC3) were negatively enriched in ABEVs compared to brainstem lysates. (B) All proteins were normalized to total protein stain as a loading control. (C) Quantification of normalized protein levels from A. All p -values are derived from two-tailed Student's t -tests except for procaspase-3, for which Welch's heteroscedastic t -test was necessary. (D) Proportionally accurate Venn diagram of proteins identified in each ABEV replicate. The substantial triple-overlap reflects a high degree of conservation among the proteomes of the individual ABEV replicates. (E) Comparison of ExoCarta dataset counts of brainstem proteins and ABEV proteins found in one, two, or three replicates. The number of EV proteomic datasets containing each protein on the EV database ExoCarta ("ExoCarta dataset counts") was used to estimate the frequency that proteins are found in EVs. ABEV proteins had higher ExoCarta dataset counts than brainstem proteins regardless

ApoA1 Antibody in Western Blot (WB) — Published figure using ApoA1 polyclonal antibody (Product # PA5-21166) in Western Blot

Immunogen image

ApoA1 Polyclonal Antibody

Immunogen Image ApoA1 Polyclonal Antibody

产品信息

PA5-21166

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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产品规格

种属反应

Chicken, Human

已发表种属

Chicken, Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A 19 amino acid peptide near the carboxy terminus of human ApoA1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

A suggested positive control is chicken liver lysate.

PA5-21166 can be used with blocking peptide PEP-1280.

靶标信息

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 1-Sep; 2-Sep; Apo a; apo A-1; APO A1; APO I; Apo-AI; apoA-I; ApoAI; Apolipoprotein; Apolipoprotein A-I; apolipoprotein A-I precursor; Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; prepro-apolipoprotein-AI; Udpgt; Ugt1; Ugt1a7

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基因别名: apo(a); APOA1

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UniProt ID: (Chicken) P08250, (Human) P02647

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Entrez Gene ID: (Chicken) 396536, (Human) 335

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beta-amyloid binding protein binding phospholipid binding high-density lipoprotein particle binding cholesterol binding enzyme binding heat shock protein binding phosphatidylcholine binding apolipoprotein A-I receptor binding identical protein binding chemorepellent activity phosphatidylcholine-sterol O-acyltransferase activator activity high-density lipoprotein particle receptor binding intermembrane cholesterol transfer activity phospholipid transporter activity cholesterol transporter activity apolipoprotein receptor binding lipase inhibitor activity apolipoprotein

regulation of protein phosphorylation endothelial cell proliferation phosphatidylcholine biosynthetic process cholesterol biosynthetic process G-protein coupled receptor signaling pathway integrin-mediated signaling pathway glucocorticoid metabolic process negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol esterification positive regulation of cholesterol efflux positive regulation of triglyceride catabolic process negative regulation of very-low-density lipoprotein particle remodeling protein oxidation peptidyl-methionine modification lipid storage regulation of intestinal cholesterol absorption adrenal gland development regulation of Cdc42 protein signal transduction cholesterol efflux phospholipid efflux negative regulation of heterotypic cell-cell adhesion very-low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly positive regulation of Rho protein signal transduction lipoprotein metabolic process lipoprotein biosynthetic process cholesterol homeostasis blood vessel endothelial cell migration reverse cholesterol transport myoblast differentiation positive regulation of fatty acid biosynthetic process phosphatidylcholine metabolic process positive regulation of lipid biosynthetic process negative regulation of inflammatory response positive regulation of phagocytosis protein stabilization negative chemotaxis positive regulation of lipoprotein lipase activity vitamin transport negative regulation of hydrolase activity positive regulation of stress fiber assembly regulation of cell cycle phospholipid homeostasis negative regulation of cell adhesion molecule production triglyceride homeostasis cholesterol import intermembrane lipid transfer positive regulation of substrate adhesion-dependent cell spreading negative regulation of lung ciliated cell differentiation positive regulation of phospholipid efflux retinoid metabolic process platelet degranulation negative regulation of cytokine secretion involved in immune response phospholipid metabolic process receptor-mediated endocytosis transforming growth factor beta receptor signaling pathway response to nutrient cholesterol metabolic process peripheral nervous system axon regeneration triglyceride catabolic process cholesterol transport organ regeneration neuron projection regeneration high-density lipoprotein particle clearance response to drug response to estrogen cellular protein metabolic process negative regulation of interleukin-1 beta secretion positive regulation of hydrolase activity transmembrane transport negative regulation of lipase activity negative regulation of response to cytokine stimulus ERK1 and ERK2 cascade