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Rockland
Asap1 Polyclonal Antibody
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Asap1 Antibody (600-401-911) in ICC/IF
Immunofluorescent microscopy using Rocklands Affinity Purified anti-ASAP1 antibody shows detection of ASAP1 present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
产品信息
600-401-911
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Dog,
Chicken,
Chimpanzee,
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of mouse ASAP1 protein.
偶联物
形式
Liquid
浓度
0.95 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against mouse ASAP1 protein. Reactivity occurs against both the phosphorylated and non-phosphorylated forms of the protein at residue Y782. A BLAST analysis was used to suggest cross reactivity with ASAP1 proteins from human, chicken, bovine, dog, rat and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
靶标信息
ASAP1, also known as Development and differentiation enhancing factor 1, is a protein with two ANK repeats, an Arf-GAP domain, a PH domain and a SH3 domain. ASAP1 is a homodimer known to interact with SRC and CRK and its activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2). It possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. It may coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2 and may also function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. It is widely expressed in most of the tissues. ASAP1 is a protooncogene involved in invasive breast cancer and in other types of invasive and malignant tumors, such as uveal melanomas.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein; 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; ADP-ribosylation factor-directed GTPase-activating protein 1; ARF GTPase-activating protein 1; Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; centaurin, beta 4; DEF-1; development and differentiation enhancing factor 1; Development and differentiation-enhancing factor 1; differentiation-enhancing factor 1; PIP2-dependent ARF1 GAP; src SH3 binding protein
基因别名: AMAP1; ASAP1; AV239055; CENTB4; DDEF1; DEF-1; KIAA1249; mKIAA1249; PAG2; PAP; s19; Shag1; ZG14P
UniProt ID: (Bovine) O97902, (Human) Q9ULH1, (Mouse) Q9QWY8, (Rat) Q1AAU6
Entrez Gene ID: (Bovine) 327705, (Chicken) 428385, (Dog) 482044, (Human) 50807, (Mouse) 13196, (Rat) 314961
