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Invitrogen
Asporin Polyclonal Antibody
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Asporin Antibody (PA5-87799) in WB
Western blot analysis of Asporin in extracts of various cell lines. Samples were incubated with Asporin Polyclonal antibody (Product # PA5-87799) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
PA5-87799
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 100-379 of human ASPN (NP_0601504)
偶联物
形式
Liquid
浓度
2.56 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: PFDTRMLDLQ NNKIKEIKEN DFKGLTSLYG LILNNNKLTK IHPKAFLTTK KLRRLYLSHN QLSEIPLNLP KSLAELRIHE NKVKKIQKDT FKGMNALHVL EMSANPLDNN GIEPGAFEGV TVFHIRIAEA KLTSVPKGLP PTLLELHLDY NKISTVELED FKRYKELQRL GLGNNKITDI ENGSLANIPR VREIHLENNK LKKIPSGLPE LKYLQIIFLH SNSIARVGVN DFCPTVPKMK KSLYSAISLF NNPVKYWEMQ PATFRCVLSR MSVQLGNFGM; Positive Samples: HeLa, SKOV3, SGC-7901, Mouse heart, Mouse stomach, Rat heart; Cellular Location: extracellular matrix, extracellular space
靶标信息
ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Asporin; asporin (LRR class 1); asporin proteoglycan; FLJ20129; periodontal ligament associated protein 1; Periodontal ligament-associated protein 1; PLAP-1; small leucine-rich protein 1C
基因别名: 4631401G09Rik; AA986886; ASPN; OS3; PLAP-1; PLAP1; SLRR1C; UNQ215/PRO241
UniProt ID: (Human) Q9BXN1, (Mouse) Q99MQ4
Entrez Gene ID: (Human) 54829, (Rat) 306805, (Mouse) 66695
biological_process
bone mineralization
negative regulation of transforming growth factor beta receptor signaling pathway
transmembrane transport
negative regulation of tooth mineralization
response to fluoride
negative regulation of protein kinase activity
cytokine-mediated signaling pathway
negative regulation of JAK-STAT cascade
biomineral tissue development
