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Invitrogen
CCL6 Polyclonal Antibody, PeproTech®
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CCL6 Antibody (500-P112-1MG) in WB
Western Blot: To detect Murine C10 (CCL6) by Western Blot analysis CCL6 Polyclonal Antibody (Product # 500-P112-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine C10 (CCL6) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P112-1MG
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine C10 (CCL6)
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
产品详细信息
AA Sequence of recombinant protein: GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine C10 (CCL6). Anti-Murine C10 (CCL6)-specific antibody was purified by affinity chromatography employing an immobilized Murine C10 (CCL6) matrix.
Sandwich ELISA: To detect Murine C10 (CCL6) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine C10 (CCL6) (500-P112Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine C10 (CCL6).
Western Blot: To detect Murine C10 (CCL6) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine C10 (CCL6) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Chemokine(C-C motif) ligand 6(CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin(e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C-C motif chemokine 6; CC chemokine C10; Protein C10; small inducible cytokine A6; Small-inducible cytokine A6
基因别名: C10; Ccl6; MRP-1; Scya6
UniProt ID: (Mouse) P27784
Entrez Gene ID: (Mouse) 20305
