Invitrogen

CD141 Monoclonal Antibody (LS17-9), eBioscience™

Name: CD141 Monoclonal Antibody (LS17-9), eBioscience\u2122
Brand: Invitrogen
SKU: 14-1411-82

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1 篇参考文献

查看其他43个Thrombomodulin (CD141)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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CD141 Antibody in Immunocytochemistry (ICC/IF)

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CD141 Antibody (14-1411-82) in ICC/IF

Immunocytochemistry of fixed and permeabilized mouse bEnd-3 cells (left) or immunohistochemistry of frozen mouse lymph node (right) using 10 µg/mL of Anti-Mouse CD141 Purified, followed by Anti-Rat IgG TRITC. Nuclei are stained with DAPI.

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产品信息

14-1411-82

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

10 µg/mL

免疫细胞化学 (ICC/IF)

10 µg/mL

流式细胞分析 (Flow)

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产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

LS17-9

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2572864

产品详细信息

Description: The monoclonal antibody LS17-9 recognizes mouse CD141 (Thrombomodulin, BDCA-3), a type I cell surface glycoprotein that binds to and allosterically activates thrombin. This complex cleaves protein C and the Thrombin Activatable Fibrinolysis Inhibitor (TAFI), initiating an important anticoagulant and antifibrinolytic pathway. Although expressed across many tissues and cell types, its highest expression has been reported on the lumenal surface of vascular endothelial cell. In the immune system the highest expression can be observed on monocytes and macrophages. Soluble CD141 is constitutively present in the serum at a very low level, and can be upregulated during inflammation. The anti-inflammatory activity of CD141 results from its ability to block the highly pro-inflammatory activity of thrombin. CD141 prevents chemotaxis and binding of granulocytes to activated endothelium, and inhibits activation of eosinophils and mast cells thereby reducing inflammation. Although human CD141 is commonly used as a marker of cross-presenting dendritic cells, mouse CD141 does not exhibit the same expression pattern.

Applications Reported: This LS17-9 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, and immunocytochemistry.

Applications Tested: This LS17-9 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 10 µg/mL. This LS17-9 antibody has been tested by immunocytochemistry of methanol or formaldehyde-fixed and permeabilized mouse cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Thrombomodulin (TM) is also known as Fetomodulin (FM), endothelial anticoagulant protein, or glycoprotein P112. Thrombomodulin contains six repeated domains homologous with epidermal growth factor (EGF) and an amino terminal domain homologous to lectin-like protein. Through its accelerated activation of protein C, synthesis of TM is one of several mechanisms important in maintaining thrombo-resistance and thus reducing clot formation on the surface of the endothelial cells. Immunohistochemical staining of formalin-fixed tissue for TM has been used for the study of a variety of vascular tumors, meningiomas and choriocarcinomas.

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