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Invitrogen
CD16 Polyclonal Antibody
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CD16 Antibody (PA5-47230) in ICC/IF
Immunocytochemistry analysis of CD16 in immersion fixed mouse splenocytes. Samples were incubated in CD16 polyclonal antibody (Product # PA5-47230) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surface.
产品信息
PA5-47230
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIII Ala31-Thr215
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Fc gamma RIIIB and rhCD32 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
靶标信息
CD16, known as Fc gamma receptor III, exists in two distinct isoforms: Fc gamma RIIIA and Fc gamma RIIIB. These isoforms are encoded by the genes FCGR3A and FCGR3B, respectively, which are located on chromosome 1 and share a high degree of similarity. Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as natural killer (NK) cells, but not on T or B cells. This receptor recognizes the Fc portion of immunoglobulin G (IgG) and is involved in the clearance of immune complexes from circulation. Upon binding IgG, Fc gamma RIIIA activates signal transduction pathways that result in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation. Fc gamma RIIIB is expressed on polymorphonuclear neutrophils (PMN) as a glycosylphosphatidylinositol (GPI)-anchored form. It plays a role in similar immune functions, including cellular-mediated cytotoxicity and enhancement of virus infections. Mutations in the FCGR3A gene have been associated with Immunodeficiency 20 and linked to susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD16-2; CD16a; CD32; CD32 receptor 2; CDw32; Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc gamma receptor III; Fc gammaRIV; Fc receptor-like 3; Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma-RIIb; Fc-gamma-RIIc; Fcg receptor III; FCG2; FcgammaRIII; FcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcRII-b; FcRII-c; fcRIII; igG Fc receptor III; IgG Fc receptor III-A; low affinity immunoglobulin gamma Fc region receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; RP11-5K23.1
基因别名: 4833442P21Rik; CD16; CD16-2; FcgammaRIV; Fcgr3a; Fcgr4; FcgRIV; Fcrl3
UniProt ID: (Mouse) A0A0B4J1G0
Entrez Gene ID: (Mouse) 14131, (Mouse) 246256
antibody-dependent cellular cytotoxicity
positive regulation of type IIa hypersensitivity
positive regulation of type III hypersensitivity
positive regulation of type I hypersensitivity
serotonin secretion
phagocytosis, recognition
phagocytosis, engulfment
cell surface receptor signaling pathway
neutrophil chemotaxis
Fc-gamma receptor signaling pathway
positive regulation of tumor necrosis factor biosynthetic process
antigen processing and presentation of exogenous peptide antigen via MHC class I
mast cell activation
positive regulation of phagocytosis
regulation of immune response
NK T cell proliferation
regulation of sensory perception of pain
cellular response to lipopolysaccharide
