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Invitrogen
CD166 Polyclonal Antibody
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FIGURE: 1 / 7
CD166 Antibody (PA5-47083) in WB
Western blot analysis of CD166 lysates of mouse brain (cerebellum) tissue, rat brain (cerebellum) tissue, H4-II-E-C3 rat hepatoma cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL CD166 Polyclonal Antibody (Product # PA5-47083) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for ALCAM/CD166 at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-47083
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166 Trp28-Lys527
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) ALCAM is observed, and less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM-1 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activated leukocyte cell adhesion molecule; ALCAM; BEN; CD166; CD166 antigen; cell adhesion molecule; FLJ38514; HB2; KG-CAM; MGC71733; Protein DM-GRASP; Protein MEMD; SB-10 antigen
Gene Aliases: AI853494; ALCAM; BEN; CD166; DM-GRASP; MEMD; MuSC; SC1
UniProt ID: (Human) Q13740, (Rat) O35112, (Mouse) Q61490
Entrez Gene ID: (Human) 214, (Dog) 478550, (Rat) 79559, (Mouse) 11658
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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