Invitrogen

CD172a (SIRP alpha) Monoclonal Antibody (P84), PE, eBioscience™

Name: CD172a (SIRP alpha) Monoclonal Antibody (P84), PE, eBioscience\u2122
Brand: Invitrogen
SKU: 12-1721-82
Price: 799.00 CNY
Availability: InStock

3个已发表图片

6 篇参考文献

查看其他75个SIRP alpha (CD172a)抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)
已发表图片 (3)

CD172a (SIRP alpha) Antibody in Flow Cytometry (Flow)

图: 1 / 4

CD172a (SIRP alpha) Antibody (12-1721-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.25 µg of Rat IgG1 K Isotype Control PE (Product # 12-4301-82) (left) or 0.25 µg of Anti-Mouse CD172a (SIRP alpha) PE (right). Cells in the large scatter population were used for analysis.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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产品信息

12-1721-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看6篇已发表文章 6篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

P84

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11151506

产品详细信息

Description: This P84 monoclonal antibody reacts with mouse CD172a, also known as signal regulatory protein a (SIRPa). This cell surface glycoprotein consists of three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The ITIM domains have been demonstrated to recruit and bind the Src homology 2 domain-containing phosphatases SHP-1 and SHP-2. CD172a is expressed on monocytes, macrophages, dendritic cells, but not on T and B lymphocytes. Moreover, neurons and other tissues of the central nervous system have also been shown to express CD172a. The integrin-associated protein CD47 is the extracellular ligand for CD172a. Studies show that CD172a is involved in dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis.

This monoclonal antibody has been reported to have neutralizing activity.

Applications Reported: This P84 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This P84 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion, and plays a general role in different physiological and pathological processes. Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: brain Ig-like molecule with tyrosine-based activation motifs; brain immunological-like with tyrosine-based motifs; CD172 alpha; CD172 antigen-like family member A; inhibitory receptor SHPS-1; mSIRP-alpha1; myD-1 antigen; protein tyrosine phosphatase, non-receptor type substrate 1; SHP substrate 1; signal-regulatory protein alpha-1; sirp-alpha-1; SIRPalpha; SIRPalpha2

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基因别名: Bit; CD172a; Idd13.2; P84; Ptpns1; SHP-1; SHPS-1; SIRP

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UniProt ID: (Mouse) P97797

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Entrez Gene ID: (Mouse) 19261

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功能

protein phosphatase inhibitor activity protein binding protein phosphatase binding GTPase regulator activity protein phosphorylated amino acid binding protein binding involved in heterotypic cell-cell adhesion protein binding involved in cell-cell adhesion protein antigen binding protein tyrosine kinase binding

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