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Invitrogen
CD183 (CXCR3) Recombinant Mouse Monoclonal Antibody (CEW33D), Alexa Fluor™ Plus 488
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CD183 (CXCR3) Antibody (740160MP488) in Flow
Flow cytometry analysis of CD183 (CXCR3) Recombinant Mouse Monoclonal Antibody (CEW33D), Alexa Fluor ™ Plus 488 (Product # 740160MP488) was performed using Human PBMCs differentiated to Th1. Differentiated Th1 cells were stained with CD3 Monoclonal Antibody (UCHT1), eFluor™ 450, eBioscience™ (Product # 48-0038-42) and 0.5 µg of CD183 (CXCR3) Recombinant Mouse Monoclonal Antibody (CEW33D), Alexa Fluor ™ Plus 488 (Product # 740160MP488) (Fig. b) or left unstained (Fig. a). Viable cells were used for analysis as determined by LIVE/DEAD™ Fixab... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
740160MP488
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
CEW33D
偶联物
Alexa Fluor™ Plus 488
Alexa Fluor™ Plus 488
Alexa Fluor™ Plus 488
激发/发射光谱
493/518 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
50 µg
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD183, also known as CXCR3, is a seven-transmembrane G protein-coupled chemokine receptor that binds CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (I-TAC), which are part of the CXC chemokine subfamily. CD183 plays a crucial role in leukocyte traffic, influencing integrin activation, cytoskeletal changes, and chemotactic migration. It is expressed on NK cells, subsets of T lymphocytes, regulatory T cells (Tregs), and is preferentially found on Th1-polarized cells. CD183 is prominently expressed in effector/memory T cells and T cells in inflamed tissues, contributing to CD4 T cell responses to grafts, as evidenced by compromised allograft rejection in CXCR3 knockout mice. Chemokine binding induces rapid, short-lived cellular responses due to receptor internalization, with responsiveness restored after receptor recycling. Inhibition by Bordetella pertussis toxin suggests coupling with Gi subclass G proteins. The production of IP-10, Mig, and I-TAC in inflammatory lesions indicates CD183 role in recruiting inflammatory cells, making it a target for developing antagonists to treat inflammatory diseases. Multiple transcript variants encoding different isoforms of CD183 have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: an; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) receptor 3; chemokine (C-X-C motif) receptor 3 isoform CXCR3_1; chemokine (C-X-C motif) receptor 3 isoform CXCR3_2; chemokine receptor 3; CXC; CXC motif chemokine; CXCR3-B; G protein-coupled receptor; G protein-coupled receptor; G protein-coupled receptor 9; interferon-inducible protein 10 receptor; IP-10 receptor; IP10; IP10 receptor; Mig receptor; unnamed protein product
基因别名: CD182; CD183; CKR-L2; CMKAR3; GPR9; IP10-R; Mig-R; MigR
UniProt ID: (Human) P49682
Entrez Gene ID: (Human) 2833
negative regulation of endothelial cell proliferation
regulation of leukocyte migration
chemotaxis
inflammatory response
immune response
cell adhesion
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
positive regulation of cell proliferation
T cell chemotaxis
negative regulation of angiogenesis
calcium-mediated signaling
regulation of cell adhesion
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
positive regulation of chemotaxis
positive regulation of release of sequestered calcium ion into cytosol
cell chemotaxis
chemokine-mediated signaling pathway
negative regulation of execution phase of apoptosis
positive regulation of execution phase of apoptosis
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