Invitrogen

CD19 Recombinant Rat Monoclonal Antibody (6OMP31), Alexa Fluor™ Plus 488

Name: CD19 Recombinant Rat Monoclonal Antibody (6OMP31), Alexa Fluor\u2122 Plus 488
Brand: Invitrogen
SKU: 740156TP488

高级验证

查看其他273个CD19抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)
高级验证 (1)

CD19 Antibody in Immunocytochemistry (ICC/IF)

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CD19 Antibody (740156TP488) in ICC/IF

Immunofluorescence analysis of CD19 was performed using CD19 Recombinant Rat Monoclonal Antibody (6OMP31), Alexa Fluor™ Plus 488 (Product # 740156TP488) on 70-80% confluent Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with CD19 Recombinant Rat Monoclonal Antibody (6OMP31), Alexa Fluor™ Plus 488 (Product # 740156TP488, 1:100). Panel a) shows representative image of cells that were stained for detection and localization of CD19 (green).... View More

CD19 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

CD19 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of CD19 was observed in Raji, Ramos and Daudi when compared to Jurkat, MOLT-4 and K-562 cell models using CD19 Recombinant Rat Monoclonal Antibody (6OMP31), Alexa Fluor™ Plus 488 (Product # 740156TP488) in Western blot. {RE}

产品信息

740156TP488

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

免疫组化（石蜡） (IHC (P))

1:50

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

0.5 µg/test

产品规格

种属反应

Human

宿主/亚型

Rat / IgG2a, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

6OMP31

偶联物

Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488

激发/发射光谱

493/518 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

proprietary buffer, pH 6.3-7.3

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser

靶标信息

CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.

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