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Invitrogen
CD28 Polyclonal Antibody
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CD28 Antibody (PA5-116751) in WB
Western blot analysis of CD28 in extracts of various cell lines. Samples were incubated with CD28 Polyclonal antibody (Product # PA5-116751) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
产品信息
PA5-116751
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 180-220 of human CD28 (NP_0061301)
偶联物
形式
Liquid
浓度
0.68 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Samples: HL-60, Raji, Mouse spleen, Rat spleen, Rat kidney, Rat thymus
Immunogen sequence: RSKRSRLLHS DYMNMTPRRP GPTRKHYQPY APPRDFAAYR S
靶标信息
CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD28; CD28 antigen; MGC138290; sCD28; soluble CD28; T-cell-specific surface glycoprotein CD28; T44; TP44
基因别名: CD28; CD28RNA; Tp44
UniProt ID: (Human) P10747, (Mouse) P31041, (Rat) P31042
Entrez Gene ID: (Human) 940, (Mouse) 12487, (Rat) 25660
positive regulation of inflammatory response to antigenic stimulus
humoral immune response
cell surface receptor signaling pathway
positive regulation of gene expression
negative regulation of gene expression
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
T cell costimulation
positive regulation of interleukin-10 production
positive regulation of interleukin-4 production
cytokine biosynthetic process
positive regulation of T cell proliferation
negative thymic T cell selection
regulatory T cell differentiation
positive regulation of viral genome replication
positive regulation of interleukin-2 biosynthetic process
regulation of regulatory T cell differentiation
positive regulation of translation
positive regulation of mitotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
positive regulation of alpha-beta T cell proliferation
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of isotype switching to IgG isotypes
regulation of defense response to virus by virus
T cell receptor signaling pathway
positive regulation of protein kinase B signaling
apoptotic signaling pathway
immune response
positive regulation of signal transduction
T cell activation
signal transduction
