Leinco Technologies

CD3 epsilon Monoclonal Antibody (145-2C11), Functional Grade, In Vivo PLATINUM™

Name: CD3 epsilon Monoclonal Antibody (145-2C11), Functional Grade, In Vivo PLATINUM\u2122
Brand: Leinco Technologies
SKU: C2280-1MG

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说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

Immunogen image

Immunogen Image CD3 epsilon Monoclonal Antibody (145-2C11), Functional Grade, In Vivo PLATINUM™

产品信息

C2280-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

流式细胞分析 (Flow)

Assay-dependent

功能检测 (Functional)

Assay-dependent

抑制试剂 (Inhibition)

Assay-dependent

T细胞活化 (TCA)

Assay-dependent

产品规格

种属反应

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

145-2C11

抗原

H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37

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偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

≥5.0 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.2-7.4

内含物

no preservative

保存条件

4°C short term, -80°C long term

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Specificity: Clone 145-2C11 recognizes an epitope on mouse CD3e.

Endotoxin Level: ≤ 0.5 EU/mg as determined by the LAL method. Purity: >95% by SDS Page; ≥98% monomer by analytical SEC.

Product Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing: To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8 C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80 C. Avoid Repeated Freeze Thaw Cycles.

This antibody has been tested in the following applications: Western Blotting, Immunohistochemistry-Fresh Frozen, Immunofluorescence, Immunocytochemistry, Functional Assay, Flow Cytometry, Depletion, CyTOF® CODEX® Blocking, Activation.

靶标信息

The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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基因别名: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz