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Invitrogen
CD307d (FcRL4) Monoclonal Antibody (413D12), PerCP-eFluor™ 710, eBioscience™
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CD307d (FcRL4) Antibody (46-3079-42) in Flow
Staining of normal human peripheral blood cells (left) and human tonsil cells (right) with Anti-Human CD19 eFluor® 450 (Product # 48-0198-42) and Anti-Human CD307d (FcRL4) PerCP-eFluor® 710 (Product # 46-3079-42). Cells in the lymphocyte gate were used for analysis. Data courtesy of the laboratory of Shane Crotty.
产品信息
46-3079-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
413D12
偶联物
激发/发射光谱
482/708 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The 413D12 monoclonal antibody reacts with human CD307d, which is also known as FcRL4, Fc receptor-like 4, FcRH4, IRTA4, or IGFP2. CD307d is an inhibitory receptor that is highly expressed on a subset of memory B cells resident in epithelial tissues and marginal zones of mucosal lymphoid tissues such as tonsils. CD307d has been shown to recruit the phosphatase SHP-1, thereby acting as an inhibitory receptor in B cells. CD307d is homologous to FcgammaRI and specifically binds IgA. To date, CD307d is the only known inhibitory receptor for IgA. Expression of CD307d is increased on memory B cells from patients with chronic infections such as HIV, suggesting that enhanced expression of inhibitory receptors, such as CD307d, may contribute to the inefficiency of antibody responses against HIV.
Applications Reported: This 413D12 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 413D12 antibody has been pre-titrated and tested by flow cytometric analysis of human tonsil tissue or transfected 293T cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains three immune-receptor tyrosine-based inhibitory motifs. This protein may play a role in the function of memory B-cells in the epithelia. Aberrations in the chromosomal region encoding this gene are associated with non-Hodgkin lymphoma and multiple myeloma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD307d; Fc receptor homolog 4; Fc receptor-like 4; Fc receptor-like protein 4; FcR-like protein 4; FcRH4; hIFGP2; IFGP family protein 2; Immune receptor translocation-associated protein 1; immunoglobulin superfamily Fc receptor, gp42; immunoglobulin superfamily receptor translocation associated 1; MGC150522; MGC150523
基因别名: CD307d; FCRH4; FCRL4; IFGP2; IGFP2; IRTA1
UniProt ID: (Human) Q96PJ5
Entrez Gene ID: (Human) 83417
