Invitrogen

CD370 (Clec9A) Monoclonal Antibody (42D2), PerCP-eFluor™ 710, eBioscience™

Name: CD370 (Clec9A) Monoclonal Antibody (42D2), PerCP-eFluor\u2122 710, eBioscience\u2122
Brand: Invitrogen
SKU: 46-5975-82

3个已发表图片

2 篇参考文献

查看其他17个CLEC9A抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (3)

CD370 (Clec9A) Antibody in Flow Cytometry (Flow)

图: 1 / 4

CD370 (Clec9A) Antibody (46-5975-82) in Flow

Staining of SJL splenocytes with Anti-Mouse CD317 (BST2, PDCA-1) eFluor® 450 (Product # 48-3172-82) and 0.5 µg of Rat IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4301-80) (left) or 0.5 µg of Anti-Mouse CD370 (Clec9A) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

46-5975-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Mouse

已发表种属

C. elegans, Mouse

宿主/亚型

Rat / IgG1

分类

Monoclonal

类型

Antibody

克隆号

42D2

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10717291

产品详细信息

Description: This 42D2 monoclonal antibody reacts with mouse CD370, which is also known as DNGR-1 or Clec9A. CD370 is a C-type lectin-like receptor expressed on conventional and plasmacytoid dendritic cells (DCs). In particular, CD8+ conventional DCs in the thymus, spleen, and lymph nodes, as well as CD24+ DCs in the blood, express CD370. This receptor is not detectable on most B cells, T and NK cells, monocytes, and macrophages. The cytoplasmic domain of CD37° Contains an ITAM motif that has been demonstrated to phosphorylate Syk tyrosine kinase, leading to NFAT activation. This signaling reportedly mediates the presentation of necrotic cell-associated antigens by CD8+ DCs in innate immunity.

Applications Reported: This 42D2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 42D2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

C-type lectin domain family 9 member A (CLEC9A) is a gene located on chromosome 12p13.2, encoding a protein that belongs to the C-type lectin receptor family. CLEC9A is primarily expressed on dendritic cells and certain subsets of macrophages, playing a pivotal role in the immune system by recognizing and binding dead cell debris, including necrotic cells. This receptor facilitates the presentation of antigens derived from dead cells to T cells, thus contributing to the initiation of immune responses. CLEC9A is involved in cross-presentation, a process crucial for adaptive immunity, particularly in the context of viral infections and cancer. Its expression and activity are of particular interest for vaccine development and immunotherapy, given its ability to enhance antigen presentation and stimulate robust immune responses. Research into CLEC9A continues to explore its therapeutic potential for modulating immune activity in various diseases, including cancer and infectious diseases.

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