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CD4/CD28 Antibody Cocktail, FITC, PE

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CD4/CD28 Antibody Cocktail, FITC, PE

产品信息

0428S

应用
建议稀释比
已发表文章

流式细胞分析 (Flow)

10 µL/1x10^6 cells in a 100 µL suspension
-
产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Cocktail

类型

Antibody

抗原

CD4 was derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.CD28 was derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the HPB-ALL T-cell line.
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偶联物

FITC , PE FITC , PE FITC , PE

形式

Liquid

纯化类型

Protein A/G

保存液

PBS

内含物

0.08% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles, store in dark

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Flow Cytometry PBMC: Add 10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.

Flow Cytometry Whole Blood: Add10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

靶标信息

The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD28; CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; MGC138290; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell-specific homodimer surface protein precursor; T-cell-specific surface glycoprotein CD28; T4 surface glycoprotein precursor; TP44; unnamed protein product

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基因别名: CD28; CD4; CD4mut; IMD123; IMD79; Leu-3; OKT4D; T4; Tp44

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UniProt ID: (Human) P10747, (Human) P01730

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Entrez Gene ID: (Human) 940, (Human) 920

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功能
protein binding coreceptor activity protein kinase binding identical protein binding virus receptor activity transmembrane signaling receptor activity receptor binding extracellular matrix structural constituent zinc ion binding lipid binding immunoglobulin binding enzyme binding MHC class II protein complex binding signaling receptor activity interleukin-16 binding interleukin-16 receptor activity MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding
参与通路
positive regulation of protein phosphorylation adaptive immune response immune system process positive regulation of immune system process immune response cell adhesion signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol calcium-mediated signaling T cell differentiation macrophage differentiation response to estradiol maintenance of protein location in cell positive regulation of interleukin-2 production response to vitamin D positive regulation of kinase activity helper T cell enhancement of adaptive immune response interleukin-15-mediated signaling pathway positive regulation of T cell proliferation T cell activation positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade T cell selection response to ethanol positive regulation of monocyte differentiation positive regulation of protein kinase activity positive regulation of transcription, DNA-templated positive regulation of viral entry into host cell viral entry into host cell defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation positive regulation of multicellular organismal process regulation of calcium ion transport positive regulation of ERK1 and ERK2 cascade cellular response to granulocyte macrophage colony-stimulating factor stimulus response to methamphetamine hydrochloride cellular response to ionomycin positive regulation of cytokine production positive regulation of inflammatory response to antigenic stimulus transcription from RNA polymerase II promoter humoral immune response positive regulation of gene expression negative regulation of gene expression T cell costimulation positive regulation of interleukin-10 production positive regulation of interleukin-4 production CD4-positive, alpha-beta T cell proliferation T cell proliferation regulation of T cell proliferation negative regulation of apoptotic process protein kinase B signaling negative thymic T cell selection regulatory T cell differentiation positive regulation of viral genome replication regulation of regulatory T cell differentiation positive regulation of translation positive regulation of mitotic nuclear division positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgG isotypes T cell receptor signaling pathway positive regulation of protein kinase B signaling apoptotic signaling pathway positive regulation of CD4-positive, alpha-beta T cell proliferation
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