Invitrogen

CD44H Monoclonal Antibody (OX49), PE, eBioscience™

Name: CD44H Monoclonal Antibody (OX49), PE, eBioscience\u2122
Brand: Invitrogen
SKU: 12-0444-82

6个已发表图片

17 篇参考文献

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (6)

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CD44H Antibody (12-0444-82) in Flow

Staining of rat splenocytes with Anti-Rat MHC Class II APC (Product # 17-0920-82) and 0.03 µg of Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (left) or Anti-Rat CD44H PE (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-0444-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.06 µg/test

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产品规格

种属反应

Rat

已发表种属

C. elegans, Mouse, Rat

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

OX49

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11149352

产品详细信息

Description: This OX49 monoclonal antibody recognizes rat CD44H, which is also known as homing-associated cell adhesion molecule (H-CAM) or phagocytic glycoprotein-1 (Pgp-1). This isoform of CD44 is the cell surface receptor for hyaluronate, a component of the extracellular matrix. CD44H is highly expressed on hematopoietic and non-hematopoietic cells, and exists as multiple splice variants. CD44H plays a critical role in lymphocyte activation and homing.

Applications Reported: This OX49 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX49 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

生物信息学

蛋白别名: CD44; CD44 antigen; Cell surface glycoprotein CD44 (hyaluronate binding protein); ECMR-III; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; HUTCH-I; Hyaluronate receptor; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

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基因别名: Cd44; CD44A; METAA; RHAMM

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UniProt ID: (Rat) P26051

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Entrez Gene ID: (Rat) 25406

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功能