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Invitrogen
CD45R (B220) Monoclonal Antibody (RA3-6B2), eVolve™ 605, eBioscience™
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-RA3-6B2-RE-AV-20170920122035.jpg?time=20220826)
-RA3-6B2-RE-AV-20170920122035.jpg?time=20220826?time=20220826 "CD45R (B220) Antibody (83-0452-41)")
产品信息
83-0452-41
应用
建议稀释比
已发表文章
产品规格
已发表种属
Fish
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
RA3-6B2
偶联物
eVolve™ 605
eVolve™ 605
eVolve™ 605
形式
Liquid
浓度
5 µL/Test
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
产品详细信息
Notice: The eVolve™ line of antibody conjugates will be discontinued at the end of February, 2018 and replaced with our improved Invitrogen™ eBioscience™ Super Bright conjugated antibodies for flow cytometry. Individual eVovle catalog numbers will become unavailable as inventory depletes. This item has a corresponding Super Bright 600 format available - please see Product # 63-0452-42. We will continue to offer a selection of Qdot conjugated antibodies.
Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.
Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RA3-6B2 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes. This can be used at 5 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
Laser/Filter Recommendation: eVolve™ 605 can be optimally excited with laser lines from 325-405 nm, and emits near 610 nm. We recommend using the violet (405 nm) laser line for excitation and a 595LP with a 605/40 or 610/20 bandpass filter, or equivalent, for detection.
Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.
Excitation: 350-590 nm; Emission: 605 nm; Laser: Ultraviolet Laser, Violet Laser
Storage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
靶标信息
CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.
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