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Invitrogen
CD47 Polyclonal Antibody
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CD47 Antibody (PA5-81591) in WB
Western Blot using CD47 Polyclonal Antibody (Product # PA5-81591) at 1:100 dilution. Lane A: Hela Membrane Lysate, Lane B: Jurkat Membrane Lysate. Lysates/proteins at 30 µg per lane. Secondary antibody: Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 47 kDa.
产品信息
PA5-81591
产品规格
种属反应
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Rat CD47 protein (Met1-Lys140)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Rat CD47.
靶标信息
CD47, known as integrin-associated protein (IAP), is a glycosylated transmembrane glycoprotein with five domains, expressed widely across hematopoietic cells like T and B cells, monocytes, platelets, and erythrocytes, as well as non-hematopoietic cells. It interacts with integrins such as CD51/CD61 and CD41/CD61, and serves as a receptor for thrombospondin, mediating bi-directional signaling that affects neural synaptic activity and macrophage phagocytosis. CD47 acts as a ligand for CD172a (SIRP alpha), an inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. This interaction influences cell migration, B cell adhesion, T cell activation, and neuronal development, particularly in synapse-rich brain and retina regions. It also modulates chondrocyte responses to mechanical signals. T cell expression of CD47 can lead to activation or apoptosis in the presence of thrombospondin. Monoclonal antibody stimulation of CD47 has been shown to induce CD4+CD25- suppressive activity and increase Foxp3 expression. CD47's role in membrane transport, signal transduction, and its broad tissue distribution highlight its significance in various physiological processes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); IAP; Integrin-associated protein; Leukocyte surface antigen CD47; sCD47; soluble CD 47; soluble CD47
基因别名: Cd47; Itgp
UniProt ID: (Rat) P97829
Entrez Gene ID: (Rat) 29364
cell adhesion
integrin-mediated signaling pathway
opsonization
positive regulation of cell proliferation
response to bacterium
cell migration
positive regulation of cell-cell adhesion
positive regulation of inflammatory response
positive regulation of phagocytosis
positive regulation of T cell activation
