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Invitrogen
CES3 Polyclonal Antibody
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CES3 Antibody (PA5-47802) in WB
Western blot analysis of CES3 in mouse lung tissue and rat lung tissue. Samples were incubated in CES3 polyclonal antibody (Product # PA5-47802) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Carboxylesterase 3/CES3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-47802
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Mouse myeloma cell line NS0-derived recombinant mouse Carboxylesterase3/CES3 Tyr19-Glu561
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
0.2 mg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody detects recombinant mouse Carboxylesterase 3/CES3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CES1, rhCES2, recombinant mouse (rm) CES2, and rmCES5 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
靶标信息
CES3 major lipase in white adipose tissue. Involved in the metabolism of xenobiotics and of natural substrates. Hydrolyzes triacylglycerols and monoacylglycerols, with a preference for monoacylglycerols. The susceptibility of the substrate increases with decreasing acyl chain length of the fatty acid moiety. Catalyzes the synthesis of fatty acid ethyl esters.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: carboxyesterase ES-10; carboxylesterase 3; carboxylesterase 3, pseudogene 1; EC 3.1.1; EC 3.1.1.1; ES-HVEL; Es-male carboxylesterase; ES31FLJ21736; esterase 31; FAEE synthase; fatty acid ethyl ester synthase; liver carboxylesterase 10; liver carboxylesterase 31; pI 6.1 esterase; triacylglycerol hydrolase; unnamed protein product
基因别名: Ces3; Ces3-ps1; Ces31; Es31; TGH
UniProt ID: (Mouse) Q8VCT4, (Rat) P16303, (Mouse) Q63880
Entrez Gene ID: (Mouse) 104158, (Rat) 113902, (Mouse) 382053, (Rat) 689754
sterol esterase activity
triglyceride lipase activity
fatty-acyl-ethyl-ester synthase activity
methylumbelliferyl-acetate deacetylase activity
all-trans-retinyl-palmitate hydrolase, all-trans-retinol forming activity
retinyl-palmitate esterase activity
carboxylic ester hydrolase activity
carboxylesterase activity
acyl-CoA metabolic process
response to toxic substance
lipid catabolic process
short-chain fatty acid catabolic process
very-low-density lipoprotein particle assembly
retinol metabolic process
positive regulation of transcription from RNA polymerase II promoter
acylglycerol catabolic process
circadian temperature homeostasis
cytosolic lipolysis
cellular response to cold
cold-induced thermogenesis
retinoid metabolic process
cholesterol biosynthetic process
cholesterol metabolic process
positive regulation of cholesterol efflux
negative regulation of cholesterol storage
epithelial cell differentiation
cholesterol homeostasis
reverse cholesterol transport
response to ethanol
medium-chain fatty acid metabolic process
regulation of bile acid biosynthetic process
cellular response to cholesterol
cellular response to low-density lipoprotein particle stimulus
positive regulation of cholesterol metabolic process
regulation of bile acid secretion
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