Invitrogen

CGRRF1 Polyclonal Antibody

查看其他8个CGRRF1抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (4)

CGRRF1 Antibody in Immunocytochemistry (ICC/IF)

CGRRF1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

CGRRF1 Antibody (PA5-103723) in ICC/IF

Immunocytochemistry analysis of CGRRF1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CGRRF1 polyclonal antibody (Product # PA5-103723) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

CGRRF1 Antibody in Immunocytochemistry (ICC/IF)

CGRRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CGRRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CGRRF1 Antibody in Western Blot (WB)

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CGRRF1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of CGRRF1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CGRRF1 polyclonal antibody (Product # PA5-103723) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

CGRRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CGRRF1 in human pancreatic cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with CGRRF1 polyclonal antibody (Product # PA5-103723) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

CGRRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CGRRF1 in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with CGRRF1 polyclonal antibody (Product # PA5-103723) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

CGRRF1 Antibody in Western Blot (WB) — Western blot analysis of CGRRF1 in extracts from Mouse brain. Samples were incubated with CGRRF1 polyclonal antibody (Product # PA5-103723). The lane on the left was treated with blocking peptide.

Immunogen image

CGRRF1 Polyclonal Antibody

Immunogen Image CGRRF1 Polyclonal Antibody

产品信息

PA5-103723

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human CGRRF1(Accession Q99675), corresponding to amino acid residues L206-K256.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total CGRRF1.

靶标信息

CGRRF1 is able to inhibit growth in several cell lines.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Cell growth regulator with RING finger domain protein 1; Cell growth regulatory gene 19 protein; RING finger protein 197

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基因别名: 1110038G02Rik; 1810009H17Rik; CGR19; CGRRF1; RNF197

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UniProt ID: (Human) Q99675, (Mouse) Q8BMJ7

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Entrez Gene ID: (Human) 10668, (Mouse) 68755

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zinc ion binding molecular_function metal ion binding

response to stress cell cycle arrest negative regulation of cell proliferation cell cycle negative regulation of cell growth