Invitrogen

CKAP5 Monoclonal Antibody (3C13)

Name: CKAP5 Monoclonal Antibody (3C13)
Brand: Invitrogen
SKU: MA5-49287
Availability: InStock

查看其他8个CKAP5抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (8)

CKAP5 Antibody in Immunocytochemistry (ICC/IF)

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CKAP5 Antibody (MA5-49287) in ICC/IF

Immunocytochemistry analysis of CKAP5 in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in CKAP5 Monoclonal antibody (Product # MA5-49287) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More

CKAP5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image CKAP5 Monoclonal Antibody (3C13)

产品信息

MA5-49287

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/10x^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

3C13

抗原

E.coli-derived human ch TOG/CKAP5 recombinant protein (Position: M1-L221).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3074945

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human U-87MG whole cell, human COLO-320 whole cell, human SW620 whole cell, human HEPG2 whole cell, human CACO-2 whole cell, rat brain tissue, mouse brain tissue. IHC: human liver cancer tissue, human rectal cancer tissue, human renal cancer tissue, rat brain tissue. ICC/IF: Hela cell. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Colonic and hepatic tumor over-expressed protein (ch-TOG) belongs to the TOG/XMAP215/Dis1 evolutionarily ancient family of microtubule-associated proteins with isoforms found in all major kingdoms of eukaryotes. This protein is required for the assembly and function of the meiotic or mitotic spindles during cell division and is localized to microtubule-organizing centers (MTOCs) during interphase or to the spindle poles and centrosomes during mitosis or cytokinesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。