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Invitrogen
COX2 Polyclonal Antibody
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COX2 Antibody (PA5-16817) in ICC/IF
Immunofluorescence analysis of COX2 was performed using 70% confluent log phase HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with COX2 Rabbit Polyclonal Antibody (PA516817) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution o... View More
产品信息
PA5-16817
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to c-terminus of rat COX2
偶联物
形式
Liquid
浓度
0.2 mg/mL
保存条件
4°C
运输条件
Wet ice
RRID
产品详细信息
PA5-16817 targets COX2 in IHC (P), IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-16817 immunogen is synthetic peptide corresponding to c-terminus of rat COX2.
靶标信息
COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
