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Invitrogen
COX5A Monoclonal Antibody (6E9B12D5)
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COX5A Antibody (459120) in ICC/IF
Immunofluorescent analysis of Complex IV in MRC5 cells using a Complex IV Monoclonal antibody (Product # 459120) at a concentration of 10 µg/mL. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized, and then labeled with Complex IV Monoclonal antibody (Product # 459120) at a concentration of 10 µg/mL. An AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody at a concentration of 1 µg/mL was used for detection.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
459120
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Mouse,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
6E9B12D5
抗原
Bovine COX5A
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Ammonium sulfate precipitation
保存液
HEPES buffered saline
内含物
0.02% sodium azide
保存条件
4°C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive control: Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells.
靶标信息
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: COX5A; cytochrome c oxidase polypeptide Va; cytochrome c oxidase polypeptide, mitochondrial; cytochrome c oxidase subunit Va; cytochrome c oxidase, subunit Va; mitochondrial cytochrome c oxidase subunit 5a; mitochondrial cytochrome c oxidase subunit Va; unnamed protein product
基因别名: CcOX; COX; COX-VA; MC4DN20; VA
UniProt ID: (Human) P20674, (Bovine) P00426, (Mouse) P12787, (Rat) P11240
Entrez Gene ID: (Human) 9377, (Bovine) 444878, (Mouse) 12858, (Rat) 252934
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