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Invitrogen
COX6A2 Polyclonal Antibody
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COX6A2 Antibody (PA5-144051) in WB
Western blot analysis of Cox6a2 using Cox6a2 antibody (Product # PA5-144051). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: rat heart tissue lysates; Lane 2: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incuba... View More
产品信息
PA5-144051
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence of mouse Cox6a2 (WMHAGHHERPEFIPYHHLR).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: rat heart tissue, mouse heart tissue. Flow: NRK cell, HEPA1-6 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: COXVIAH; Cytochrome c oxidase polypeptide VIa-heart; Cytochrome c oxidase subunit 6A2, mitochondrial; cytochrome c oxidase subunit VIa polypeptide 2 (heart); subunit VIaH (heart-type)
基因别名: Cox6a2; COX6AH; CoxVIaH; VIaH
UniProt ID: (Mouse) P43023
Entrez Gene ID: (Mouse) 12862, (Rat) 25278
