Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CPEB1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 8
CPEB1 Antibody (PA1-1100) in ICC/IF
Immunofluorescent analysis of CPEB (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CPEB polyclonal antibody (Product # PA1-1100) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated... View More
Product Details
PA1-1100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C H(545) S M E G L R H H S P L M R N Q K N(562) of human CPEB.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-1100 detects recombinant mouse and rat CPEB (cytoplasmic polyadenylation element binding protein).
PA1-1100 has been successfully used in Western blot, ICC/IF and IHC-P procedures. By Western blot, this antibody detects ~62 and kDa protein representing recombinant mouse CPEB.
The PA1-1100 immunogen is a synthetic peptide corresponding to residues C H(545) S M E G L R H H S P L M R N Q K N(562) of human CPEB. This sequence is completely conserved in mouse. The PA1-1100 immunizing peptide (Cat. # PEP-171) is available for use in neutralization and control experiments.
Target Information
This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the nuclear membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the nuclear membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CEBP; CPE-binding protein 1; CPE-BP1; CPEB; CPEB-1; Cytoplasmic polyadenylation element-binding protein 1; FLJ13203; h-CEBP; mCPEB-1; MGC34136; MGC60106
Gene Aliases: AU024112; CPE-BP1; CPEB; CPEB-1; CPEB1; h-CPEB; hCPEB-1; mCPEB
UniProt ID: (Human) Q9BZB8, (Mouse) P70166, (Rat) P0C279
Entrez Gene ID: (Human) 64506, (Mouse) 12877, (Rat) 293056
mRNA processing
translation
negative regulation of cell proliferation
positive regulation of neuron projection development
positive regulation of cell migration
cellular response to insulin stimulus
positive regulation of translation
mRNA transport
positive regulation of nitric-oxide synthase biosynthetic process
cellular response to lipopolysaccharide
cellular response to amino acid stimulus
cellular response to hypoxia
negative regulation of cytoplasmic translation
regulation of translation
synaptonemal complex assembly
negative regulation of translation
regulation of neuronal synaptic plasticity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support