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Invitrogen
CTGF Polyclonal Antibody, Biotin, PeproTech®
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CTGF Antibody (500-P252BT-1MG) in WB
Western Blot: To detect hCTGF by Western Blot analysis CTGF Polyclonal Antibody, Biotin (Product # 500-P252BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hCTGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P252BT-1MG
产品规格
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human CTGF
偶联物
Biotin
Biotin
Biotin
形式
Lyophilized
浓度
0.1-1.0 mg/mL
保存条件
-20°C
运输条件
Ambient
RRID
产品详细信息
AA Sequence of recombinant protein: GKKCIRTPKI SKPIKFELSG CTSMKTYRAK FCGVCTDGRC CTPHRTTTLP VEFKCPDGEV MKKNMMFIKT CACHYNCPGD NDIFESLYYR KMYGDMA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CTGF. Anti-Human CTGF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hCTGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human CTGF (500-P252) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hCTGF.
Western Blot: To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hCTGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P252BT-1MG will be provided as 2 x 500 µg
靶标信息
Connective Tissue Growth Factor (CTGF), also called CCN2, is a member of the CCN family. CTGF is a secreted protein produced by umbilical veins and vascular endothelial cells. CTGF possesses an Insulin-like Growth Factor (IGF)-binding domain, a thrombospondin type 1 domain, and a cysteine knot region. CTGF plays important roles in the proliferation and differentiation of chondrocytes, induces angiogenesis, and promotes cell adhesion in fibroblasts, endothelial, and epithelial cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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