Invitrogen

CYP2C11 Polyclonal Antibody

Name: CYP2C11 Polyclonal Antibody
Brand: Invitrogen
SKU: PA3-034

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5 篇参考文献

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (5)
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CYP2C11 Antibody in Immunocytochemistry (ICC/IF)

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CYP2C11 Antibody (PA3-034) in ICC/IF

Immunofluorescent analysis of Cytochrome P450 2C11 (green) showing staining in the membrane of H-4-II-E cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 2C11 polyclonal antibody (Product # PA3-034) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room t... View More

CYP2C11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image CYP2C11 Polyclonal Antibody

产品信息

PA3-034

应用

建议稀释比

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免疫印迹 (WB)

1:1,500

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免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫组化（冰冻） (IHC (F))

1:100

免疫细胞化学 (ICC/IF)

1:20-1:200

酶联免疫吸附实验 (ELISA)

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产品规格

种属反应

Cat, Human, Rat

已发表种属

Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptides corresponding to residues D(49) I G Q S I K K F S K V(60) and Q(491) R A D S L S S H L(500) of rat Cytochrome P450 2C11.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

whole serum, PBS

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_325981

产品详细信息

PA3-034 detects cytochrome P450 2C11 (P450 2C11) from human, feline and rat tissues.

PA3-034 has been successfully used in Western blot, ICC/IF and immunohistochemical (frozen and paraffin) procedures. By Western blot, this antibody detects an ~50 kDa protein representing P450 2C11 from rat liver extract. Immunohistochemical staining of P450 2C11 in rat brain with PA3-034 results in perivascular staining.

The PA3-034 immunogen is a synthetic peptides corresponding to residues D(49) I G Q S I K K F S K V(60) and Q(491) R A D S L S S H L(500) of rat Cytochrome P450 2C11.

靶标信息

The cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA's which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). EET's, which exhibit vasodilation activity, are formed when an epoxide group is inserted between the unsaturated carbons of AA in positions 5,6; 8,9; 11,12; 14,15. EET's are produced in cerebral cortical tissue, coronary arteries and vascular endothelium. EET's are converted from AA by the 2C11 family of P450's whose expression is induced by testosterone and is therefore not generally found in females.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CYP; CYPIIC11; Cytochrome P-450(M-1); Cytochrome P450 2C11; cytochrome P450, 2c29; cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase); Cytochrome P450-UT-2; Cytochrome P450-UT-A; Cytochrome P450H; P-450(M-1); P450; P450-UT-A; P450H; UT-2

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基因别名: Cyp2c; Cyp2c-11; Cyp2c11; CYP2CII

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UniProt ID: (Rat) P08683

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Entrez Gene ID: (Rat) 29277

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功能