Invitrogen

CaV1.1 Monoclonal Antibody (1A)

Name: CaV1.1 Monoclonal Antibody (1A)
Brand: Invitrogen
SKU: MA3-920

高级验证

29个已发表图片

82 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

CaV1.1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CaV1.1 Antibody (MA3-920) in IHC (P)

Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monocl... View More

CaV1.1 Antibody in Flow Cytometry (Flow)

CaV1.1 Antibody in Immunohistochemistry (IHC)

CaV1.1 Antibody in Immunocytochemistry (ICC/IF)

CaV1.1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

CaV1.1 Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

CaV1.1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

CaV1.1 Antibody in Immunomicroscopy (IM)

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Immunogen image

Immunogen Image CaV1.1 Monoclonal Antibody (1A)

产品信息

MA3-920

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

查看42篇已发表文章 42篇已发表文章

免疫组化 (IHC)

查看22篇已发表文章 22篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

免疫组化（冰冻） (IHC (F))

1:200

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看15篇已发表文章 15篇已发表文章

流式细胞分析 (Flow)

1 µg/test

免疫沉淀 (IP)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

免疫电镜技术 (IM)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Guinea pig, Human, Mouse, Rabbit, Rat

已发表种属

C. elegans, Fish, Human, Mouse, Non-human primate, Rabbit, Rat, Zebrafish

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

抗原

Purified rabbit muscle T-tubule dihydropyridine receptor.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2069575

产品详细信息

MA3-920 detects 1,4-dihydropyridine (DHP) receptor alpha-1 subunit in human, rat, mouse, guinea pig and rabbit skeletal muscle. The DHP Receptor alpha-1 protein is also known as CACNA1S or Cav1.1 alpha-1 subunit.

MA3-920 has been successfully used in Western blot, FACS, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~200 kDa protein representing the DHP receptor in rat skeletal muscle extracts. Immunohistochemical staining of DHP receptor in rabbit skeletal muscle with MA3-920 results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines. This product can be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells.

The MA3-920 antigen is purified rabbit muscle T-tubule DHP receptor.

靶标信息

Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: calcium channel, L type, alpha 1 polypeptide, isoform 3 (skeletal muscle, hypokalemic periodic paralysis); Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle; calcium channel, voltage-dependent, L type, alpha 1S subunit; Cav1.1 alpha-1; DHP; DHPR; DHPR alpha1s; dihydropyridine calcium channel receptor protein; Dihydropyridine receptor; dihydropyridine receptor alpha 1 subunit; dihydropyridine receptor alpha 1S; Dihydropyridine receptor alpha-1S subunit; dihydropyridine-sensitive L-type calcium channel alpha-1 subunit; muscle dysgenesis; ROB1; unnamed protein product; Voltage-dependent L-type calcium channel subunit alpha-1S; Voltage-gated calcium channel subunit alpha Cav1.1

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基因别名: AW493108; CACH1; Cach1b; CACN1; CACNA1S; CACNL1A3; Cav1.1; CCHL1A3; DHPR; fmd; HOKPP; HOKPP1; hypoPP; mdg; MHS5; sj; TTPP1