Alomone Labs, LTD

Calnexin Polyclonal Antibody, Atto™ 594

Name: Calnexin Polyclonal Antibody, Atto\u2122 594
Brand: Alomone Labs, LTD
SKU: ACS-009-AR-50UL

查看其他36个Calnexin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

Calnexin Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Calnexin Antibody (ACS-009-AR-50UL) in IHC (F)

Multiplex staining of Calnexin and Presenilin-1 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse hippocampal sections using Anti-Presenilin-1-ATTO Fluor-488 Antibody (#AIP-011-AG), (1:60) and Anti-Calnexin-ATTO Fluor-594 Antibody (#ACS-009-AR), (1:60). A. Calnexin staining (red) appears in neuronal profiles. B. Presenilin-1 staining (green) in the same section appears in n... View More

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Immunogen image

Immunogen Image Calnexin Polyclonal Antibody, Atto™ 594

产品信息

ACS-009-AR-50UL

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

1:60

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

(C)KAEEDEILNRSPRNRK, corresponding to amino acid residues 572-587 of rat Canx, Intracellular, C-terminus

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偶联物

Atto™ 594 Atto™ 594 Atto™ 594

激发/发射光谱

602/622 nm

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitution: 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

靶标信息

Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

仅用于科研。不用于诊断过程。未经明确授权不得转售。