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Invitrogen
Calpain 2 Polyclonal Antibody
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Calpain 2 Antibody (PA5-27720) in ICC/IF
Immunofluorescence analysis of CAPN2 was performed using A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Calpain 2 Polyclonal Antibody (Product # PA5-27720) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Go... View More
产品信息
PA5-27720
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 162 and 383 of Human Calpain 2
偶联物
形式
Liquid
浓度
1 mg/mL
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
Recommended positive controls: Jurkat, NIH-3T3.
Predicted reactivity: Mouse (89%), Rat (89%), Pig (92%), Rabbit (92%), Chicken (85%), Sheep (93%), Rhesus Monkey (98%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
