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Invitrogen
Calreticulin Polyclonal Antibody
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FIGURE: 1 / 10
Calreticulin Antibody (PA1-903) in WB
Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), HeLa (Lane 3), K-562 (Lane 4), Jurkat (Lane 5) and A549 (Lane 6). The blot was probed with Chicken Anti-Calreticulin Polyclonal Antibody (Product # PA1-903, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate (Product # A16054, 0.4 µg/mL, 1:2500 dilution). A 55 kDa band corresponding to Calreticulin was observed across the cell lines tested. Known quantity... View More
Product Details
PA1-903
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Hamster,
Human,
Mouse,
Rabbit,
Rat
Published species
Amphibian,
Dog,
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: D(399) E K E E D E E E S P G Q A K D(414).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-903 detects calreticulin from human, rat, mouse, hamster, rabbit and canine tissues.
PA1-903 has been successfully used in Western blot, immunoprecipitation, immunocytochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa protein representing calreticulin and an ~120 kDa unidentified protein from rat brain extract.
PA1-903 immunizing peptide corresponds to amino acid residues 399-414 from mouse calreticulin. PA1-903 immunizing peptide (Cat. # PEP-017) is available for use in neutralization and control experiments.
Target Information
Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Autoantigen RO; CABP3; CALBP; Calcium-binding protein 3; Calregulin; Calreticulin; calreticulin precursor; CRP55; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin)
Gene Aliases: CALR; Calregulin; cC1qR; CRP55; CRT; CRTC; ERp60; HACBP; HEL-S-99n; I79_008346; RO; SSA
UniProt ID: (Human) P27797, (Rabbit) P15253, (Chinese hamster) Q8K3H7, (Mouse) P14211, (Rat) P18418
Entrez Gene ID: (Rabbit) 100350417, (Human) 811, (Rabbit) 100009050, (Dog) 476694, (Chinese hamster) 100689096, (Mouse) 12317, (Rat) 64202
complement component C1q binding
glycoprotein binding
DNA binding
mRNA binding
integrin binding
iron ion binding
calcium ion binding
protein binding
zinc ion binding
carbohydrate binding
ubiquitin protein ligase binding
peptide binding
hormone binding
protein binding involved in protein folding
poly(A) RNA binding
androgen receptor binding
unfolded protein binding
chaperone binding
metal ion binding
chaperone
negative regulation of transcription from RNA polymerase II promoter
antigen processing and presentation of peptide antigen via MHC class I
peptide antigen assembly with MHC class I protein complex
regulation of transcription, DNA-templated
protein folding
protein export from nucleus
cellular calcium ion homeostasis
receptor-mediated endocytosis
cell cycle arrest
spermatogenesis
positive regulation of cell proliferation
positive regulation of gene expression
negative regulation of translation
protein maturation by protein folding
cortical actin cytoskeleton organization
response to estradiol
negative regulation of intracellular steroid hormone receptor signaling pathway
response to testosterone
protein localization to nucleus
protein folding in endoplasmic reticulum
ATF6-mediated unfolded protein response
regulation of meiotic nuclear division
response to drug
glucocorticoid receptor signaling pathway
regulation of apoptotic process
negative regulation of neuron differentiation
positive regulation of DNA replication
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
negative regulation of retinoic acid receptor signaling pathway
positive regulation of phagocytosis
protein stabilization
sequestering of calcium ion
cardiac muscle cell differentiation
chaperone-mediated protein folding
cellular response to lithium ion
cellular senescence
positive regulation of substrate adhesion-dependent cell spreading
positive regulation of dendritic cell chemotaxis
response to organic substance
cellular response to organic substance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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